E. coli: red  tight junctions: blue

E. coli causing diarrhea

From the Wellcome Image Awards:

This pair of images demonstrates how a disease-causing strain of E. coli bacteria brings about diarrhoea by breaking down the waterproof barriers between the cells.

First of all the bacteria, seen as small red dots, attach to the surface of intestinal cells, making tiny pedestals out of one of the cell’s own proteins (bright green). Once attached, the bacteria send signals into the cells, causing the tight junctions (blue) between the cells to break down. Water is then able to seep out between the cells into the intestine, leading to diarrhoea.

The image on the top shows an early stage in the process where the tight junctions are still intact and show as continuous blue lines between the cells. The image on the bottom is taken later in the process, once the tight junctions have broken down. Their remnants appear as blue dots.

-image by Stephanie Schuller

These elegant micrographs are winners of the 2008 Wellcome Image Awards. View the rest of the winners in their image award gallery.

[via Biomedicine on Display]